Accession Number : ADA519002


Title :   Single Assay for Simultaneous Detection and Differential Identification of Human and Avian Influenza Virus Types, Subtypes, and Emergent Variants


Descriptive Note : Journal article


Corporate Author : NAVAL HEALTH RESEARCH CENTER SAN DIEGO CA DEPT OF RESPIRATORY DISEASE RESEARCH


Personal Author(s) : Metzgar, David ; Myers, Christopher A ; Russell, Kevin L ; Faix, Dennis ; Blair, Patrick J ; Brown, Jason ; Vo, Scott ; Swayne, David E ; Thomas, Colleen ; Stenger, David A ; Lin, Baochuan ; Malanoski, Anthony P ; Wang, Zheng ; Blaney, Kate M ; Long, Nina C ; Schnur, Joel M ; Saad, Magdi D ; Borsuk, Lisa A ; Lichanska, Agnieszka M ; Lorence, Matthew C


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a519002.pdf


Report Date : Feb 2010


Pagination or Media Count : 19


Abstract : For more than four decades the cause of most type A influenza virus infections of humans has been attributed to only two viral subtypes, A/H1N1 or A/H3N2. In contrast, avian and other vertebrate species are a reservoir of type A influenza virus genome diversity, hosting strains representing at least 120 of 144 combinations of 16 viral hemagglutinin and 9 viral neuraminidase subtypes. Viral genome segment reassortments and mutations emerging within this reservoir may spawn new influenza virus strains as imminent epidemic or pandemic threats to human health and poultry production. Traditional methods to detect and differentiate influenza virus subtypes are either time-consuming and labor-intensive (culture-based) or remarkably insensitive (antibody-based). Molecular diagnostic assays based upon reverse transcriptase-polymerase chain reaction (RT-PCR) have short assay cycle time, and high analytical sensitivity and specificity. However, none of these diagnostic tests determine viral gene nucleotide sequences to distinguish strains and variants of a detected pathogen from one specimen to the next. Decision-quality, strain- and variant-specific pathogen gene sequence information may be critical for public health, infection control, surveillance, epidemiology, or medical/veterinary treatment planning. The Resequencing Pathogen Microarray (RPM-Flu) is a robust, highly multiplexed and target gene sequencing-based alternative to both traditional culture- or biomarker-based diagnostic tests. RPM-Flu is a single, simultaneous differential diagnostic assay for all subtype combinations of type A influenza viruses and for 30 other viral and bacterial pathogens that may cause influenzalike illness. These other pathogen targets of RPM-Flu may co-infect and compound the morbidity and/or mortality of patients with influenza.


Descriptors :   *DIAGNOSIS(MEDICINE) , *STRAINS(BIOLOGY) , *INFLUENZA VIRUS , *ANTIVIRAL AGENTS , *INFECTIOUS DISEASES , *IDENTIFICATION , *DETECTION , VERTEBRATES , GENES , GENOME , PATIENTS , VIRUS DISEASES , PUBLIC HEALTH , VIRULENCE , BIRDS , PATHOGENIC MICROORGANISMS , EPIDEMIOLOGY , NUCLEOTIDES , VETERINARY MEDICINE , MEDICINE , CHROMOSOMES , MUTATIONS , HEALTH , BACTERIA , MOLECULES , TEST AND EVALUATION , SYNCHRONISM


Subject Categories : Medicine and Medical Research
      Microbiology


Distribution Statement : APPROVED FOR PUBLIC RELEASE