Accession Number : ADA515800


Title :   Identifying Breast Cancer Oncogenes


Descriptive Note : Annual summary 1 Oct 2008-30 Sep 2009


Corporate Author : DANA-FARBER CANCER INST BOSTON MA


Personal Author(s) : Shrestha, Yashaswi


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a515800.pdf


Report Date : Oct 2009


Pagination or Media Count : 7


Abstract : Breast cancer is attributed to genetic alterations, the majority of which are yet to be characterized. Oncogenic alterations that give rise to breast tumors need to be identified to develop targeted treatment options and consequently, improve clinical outcomes. We aim to identify kinases that drive breast oncogenesis. We hypothesize that a kinase gain-of-function screen in human mammary epithelial cells will identify novel breast cancer oncogenes and provide potential targets for drug intervention. The study is based on a transformation model that requires simultaneous activation of the PI3K/AKT and MEK/ERK pathways to transform human mammary epithelial cells. A pBabe-Puro-Myr-Flag kinase ORF library was screened in immortalized human mammary epithelial cells expressing myr-AKT. Three kinases PTK6, PAK1 and CAMK4 promoted robust anchorage-independent growth in soft agar and are further being validated to understand their mechanism of action and relevance in human cancer.


Descriptors :   *BREAST CANCER , ONCOGENESIS , MODELS , HUMANS , NEOPLASMS , TARGETS , TRANSFORMATIONS(MATHEMATICS) , DRIVES , SYNCHRONISM , DRUGS , INTERVENTION , CANCER , MAMMARY GLANDS , AGAR , PHOSPHORUS TRANSFERASES , ONCOGENIC VIRUSES , ACTIVATION , EPITHELIUM


Subject Categories : Anatomy and Physiology
      Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE