Accession Number : ADA515416


Title :   Phosphorus Physiology of the Marine Cyanobacterium Trichodesmium


Descriptive Note : Doctoral theses


Corporate Author : MASSACHUSETTS INST OF TECH CAMBRIDGE


Personal Author(s) : Orchard, Elizabeth D


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a515416.pdf


Report Date : Feb 2010


Pagination or Media Count : 132


Abstract : Primary producers play a critical role in the oceanic food chain and the global cycling of carbon. The marine diazotroph Trichodesmium is a major contributor to both primary production and nitrogen fixation in the tropical and subtropical oceans. These regions are often characterized by low phosphorus (P) concentrations, and P deficiency of Trichodesmium could limit growth, and constrain nitrogen fixation by this genus. Three key components of P metabolism in cyanobacteria include: inorganic phosphate (Pi) uptake, dissolved organic phosphorus (DOP) hydrolysis, and polyphosphate (polyP) biosynthesis and catabolism. These three pathways lay the foundation for the four chapters that make up the body of this thesis. Chapter two examines the relative importance of Pi and DOP in Trichodesmium in the Sargasso Sea by measuring uptake rates and uptake kinetics of 33Pi and DOP (using alpha labeled adenosine-5'-triphosphate 33P-ATP as a model P ester compound). The in situ uptake rates suggest that the contribution of P ester to total P uptake can be greater than 25% for Trichodesmium, and therefore P esters are important for supplementing Trichodesmium's P demand. Chapter three confirms the presence of polyP in the Sargasso Sea populations. This is the first detection of polyP in an oligotrophic system and may indicate micro-scale variability in DIP or DOP supply. Chapter four examines the regulation of several genes involved in P scavenging in cultures of Trichodesmium erythraeum IMS101: two copies of a high affinity phosphate binding protein (pstS and sphX) and two putative alkaline phosphatases (phoA and phoX). Chapter five calibrates the expression of phoX to decreases in nitrogen fixation rate in Trichodesmium cultures allowing the use of phoX expression as a molecular indicator of P limitation of nitrogen fixation. Detection of phoX expression in the North Atlantic and South Pacific identified the extent and magnitude of P limitation in these regions.


Descriptors :   *PHYTOPLANKTON , SARGASSO SEA , THESES , CARBON , NUTRIENTS , NITROGEN , MARINE BIOLOGY , GENES , PHYSIOLOGY , TROPICAL REGIONS , OCEANS , CARBON DIOXIDE , ESTERS , PHOSPHORUS , DISSOLVING , SUBTROPICAL REGIONS , FOOD CHAINS , BACTERIA , BIOCHEMISTRY


Subject Categories : Biology


Distribution Statement : APPROVED FOR PUBLIC RELEASE