Accession Number : ADA515336


Title :   Mitochondrial DNA Mutations in Epithelial Ovarian Tumor Progression


Descriptive Note : Annual rept. 1 Dec 2006-30 Nov 2007


Corporate Author : MOREHOUSE SCHOOL OF MEDICINE ATLANTA GA


Personal Author(s) : Aikhionbare, Felix O


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a515336.pdf


Report Date : Dec 2007


Pagination or Media Count : 19


Abstract : Early diagnosis is one of the greatest challenges facing clinicians in the treatment of epithelial ovarian cancer. As a result of mitochondria role in apoptosis and ROS, it have been implicated in carcinogenesis. In this study we detected sequence variants in two fragments of mitochondrial DNA obtained from 68 epithelia ovarian cancer tissues spanning 5317 to 7608 and 8282 to 10110 base pair, including NADH subunits 2, 3, CO I,II,III, part of ATPase 6 and several tRNA genes. MtDNA variants were obtained by using methods of RFLP and PCR-based sequence and analyzed in relationship to ovarian tumor subtypes/stages, ages and races of the patients. Thirty-nine polymorphisms/mutations were detected of which 28 were unreported. Two variant C7256T and G7520A showed a frequency of 45% (5/11) in endometriod stage III while has no incidence in serous or mucinous subtype stage III was observed. An unreported polymorphism at T8548G in ATP6 gene was detected at a high frequency of 92% in ovarian serous subtype tissues in stages II-IV. In addition, an unreported mutation at np C7520T in tRNA gene occurred in 73% African American and 27% white samples. Interestingly, variants C7020T (56%) and at np A8860G (92%) were evenly distributed in all three studied ovarian tumor subtype and stages. Perhaps, it is tempting to speculate that variant A8860G that we observed in our study may play a potential role in the onset and progression of epithelial ovarian tumor subtypes and stages.


Descriptors :   *APOPTOSIS , *OVARIAN CANCER , *EPITHELIUM , *MITOCHONDRIA , VARIATIONS , DIAGNOSIS(GENERAL) , GENES , PATIENTS , CARCINOGENS , FRAGMENTS , ADENOSINE PHOSPHATES , ENDOMETRIOSIS , OVARIES , SEQUENCES , MUTATIONS , DEOXYRIBONUCLEIC ACIDS , ONCOGENESIS , NEOPLASMS


Subject Categories : Biochemistry
      Anatomy and Physiology
      Medicine and Medical Research
      Medical Facilities, Equipment and Supplies
      Test Facilities, Equipment and Methods


Distribution Statement : APPROVED FOR PUBLIC RELEASE