Accession Number : ADA512469


Title :   Affinity Probe Capillary Electrophoresis Evaluation of Aptamer Binding to Campylobacter jejuni Bacteria


Descriptive Note : Progress rept. 2007-2008


Corporate Author : ARMY RESEARCH LAB ADELPHI MD


Personal Author(s) : Stratis-Cullum, Dimitra N ; McMasters, Sun ; Pellegrino, Paul M


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a512469.pdf


Report Date : Nov 2009


Pagination or Media Count : 22


Abstract : In this work, we developed a capillary electrophoresis immunoassay to evaluate aptamer binding to bacterial cells. This work is the first known demonstration of aptamer affinity probe capillary electrophoresis to a whole cell target. The aptamer probes investigated were developed against a Campylobacter jejuni bacterial cell target, and we evaluated binding relative to other common food-borne pathogens. Specific binding affinity was evidenced by a pronounced mobility shift and peak broadening with increasing bacteria concentration. Little to no mobility shift was observed for food-borne pathogens, Salmonella typhirium and Escherichia coli, even when increasing concentrations 10-fold over target. These results suggest that affinity probe capillary electrophoresis could be useful for qualitative screening of aptamer candidates to bacterial cell targets.


Descriptors :   *PATHOGENIC MICROORGANISMS , *JEJUNUM , *ESCHERICHIA COLI , IMMUNOASSAY , FOOD , BIOASSAY , ELECTROPHORESIS , SALMONELLA , SHIFTING , CAPILLARITY , BACTERIA


Subject Categories : Medicine and Medical Research
      Microbiology


Distribution Statement : APPROVED FOR PUBLIC RELEASE