Accession Number : ADA499664


Title :   Glutamate Receptor Aptamers and ALS


Descriptive Note : Annual rept. 8 Dec 2007-7 Dec 2008


Corporate Author : STATE UNIV OF NEW YORK AT ALBANY RESEARCH FOUNDATION


Personal Author(s) : Niu, Li


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a499664.pdf


Report Date : Jan 2009


Pagination or Media Count : 30


Abstract : Excitotoxicity is one of the leading causes for amyotrophic lateral sclerosis (ALS). Our goal was to develop a novel class of powerful aptamer-based, anti-excitotoxic inhibitors against GluR2Qflip, a key AMPA receptor subunit that controls the calcium permeability and mediates excitotoxicity. An aptamer is a single-stranded nucleic acid that directly inhibits a protein's function by folding into a specific tertiary structure that dictates high-affinity binding to the target protein. To date, we have identified two classes of aptamers (i.e. competitive and noncompetitive aptamers) against GluR2Qflip, by using a molecular biology approach called systematic evolution of ligands by exponential enrichment (SELEX). These aptamers are water soluble and have a nanomolar affinity against GluR2Qflip. Their inhibitory properties rival those of any existing small, chemical inhibitors. We are continuing to work with these aptamers towards developing them into anti-excitotoxic drugs for treating patients with ALS, including those Gulf War veterans suffering from ALS.


Descriptors :   *GLUTAMIC ACID , *RECEPTOR SITES(PHYSIOLOGY) , PERMEABILITY , WATER , PROTEINS , DISEASES , LIGANDS , MOLECULAR BIOLOGY , SALTS , INHIBITION , SENSE ORGANS , TOXINS AND ANTITOXINS , SPINAL CORD , CEREBRAL CORTEX , FUNCTIONS , GULFS


Subject Categories : Biochemistry
      Anatomy and Physiology


Distribution Statement : APPROVED FOR PUBLIC RELEASE