Accession Number : ADA495866


Title :   Modeling Thermal Inactivation of Bacillus Spores


Descriptive Note : Master's Thesis


Corporate Author : AIR FORCE INST OF TECH WRIGHT-PATTERSON AFB OH SCHOOL OF ENGINEERING AND MANAGEMENT


Personal Author(s) : Knight, Emily A


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a495866.pdf


Report Date : Mar 2009


Pagination or Media Count : 94


Abstract : This research models and analyzes methods to damage Bacillus anthracis spores through heat treatment. AFIT researchers have developed methods to characterize the effects of heating spores to high temperatures and for short durations similar to the thermal pulse of conventional weapon detonation. This research models the current experiment and evaluates the rate of thermal diffusion throughout the spores. A micro-model of the effects of dry and wet heating on a spore is presented. Heating a spore energizes adsorbed, absorbed, and chemically bound water molecules. These energized molecules have greater mobility within the spore, as well as between the spore and the surrounding environment. The water release permits hydrolysis reactions to occur with the spore's DNA and proteins. This degrades the DNA and proteins to such an extent that the DNA cannot replicate, thus causing spore death. We assert that spore damage is based on an initial DNA information content and the spore population's protein fitness. Once this protein fitness level is degraded below a critical value, the DNA cannot be repaired. A probability of kill model based on water mobility, hydrolysis, a spore's DNA information content, and the spore population's protein fitness.


Descriptors :   *PHYSICAL FITNESS , *BACILLUS ANTHRACIS , *THERMAL DIFFUSION , *HEAT TREATMENT , *DEOXYRIBONUCLEIC ACIDS , *SPORES , ANTHRAX , DEATH , ENERGETIC PROPERTIES , THERMAL RADIATION , VACCINES , HYDROLYSIS , CONVENTIONAL WARFARE , PROTEINS , HIGH TEMPERATURE , KILL PROBABILITIES , THERMAL PROPERTIES , DAMAGE


Subject Categories : Biology
      Microbiology
      Thermodynamics


Distribution Statement : APPROVED FOR PUBLIC RELEASE