Accession Number : ADA494347
Title : Fluorescence of Bacteria, Pollens, and Naturally Occurring Airborne Particles: Excitation/Emission Spectra
Descriptive Note : Progress rept.
Corporate Author : ARMY RESEARCH LAB ADELPHI MD COMPUTATIONAL AND INFORMATION SCIENCES DIRECTORATE
Personal Author(s) : Hill, Steven C ; Mayo, Michael W ; Chang, Richard K
Report Date : Feb 2009
Pagination or Media Count : 62
Abstract : The fluorescence intensity as a function of excitation and emission wavelengths (EEM spectra) was measured for different species of bacteria, biochemical constituents of cells, pollens, and vegetation. This report documents these spectra. This endeavor is part of an effort to determine useful fluorescence excitation and emission wavelengths for discrimination between biological and nonbiological particles, and among different types of biological particles. These results can assist researchers in selecting excitation and emission wavelength bands that allow partial discrimination among particle types. The EEM spectra of dry bacteria samples are different from spectra of bacteria in liquid suspensions. In the dry samples more of the emission tends to occur at longer wavelengths. We discuss appropriate excitation sources for detecting bioaerosols using autofluorescence.
Descriptors : *FLUORESCENCE , *PARTICLES , BIOCHEMISTRY , POLLEN , AIRBORNE , EMISSION SPECTRA , VEGETATION , BACTERIA , AEROSOLS , EXCITATION
Subject Categories : Optics
Air Pollution and Control
Distribution Statement : APPROVED FOR PUBLIC RELEASE