Accession Number : ADA486634


Title :   Hypoxia in Invasion and Metastasis


Descriptive Note : Final rept. 1 Aug 2006-31 Jul 2007


Corporate Author : ALBERT EINSTEIN COLL OF MEDICINE OF (YESHIVA UNIV) BRONX NY


Personal Author(s) : Wang, Weigang


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a486634.pdf


Report Date : Aug 2007


Pagination or Media Count : 9


Abstract : We ran an outstanding DOD project (W81XwH-06-1-0625) about hypoxia in invasion and metastasis by a novel technology. At first, we have successfully constructed a hypoxia-responsive fluorescence protein animal model for mammary carcinoma studying by using a mammary carcinoma cell line with constitutively expressed enhanced red fluorescence protein (RFP) as a tumor marker and green fluorescence protein (GFP) as a reporter for hypoxia and HIF- activation. The second, we identified and confirmed that increased HIF-1 expression were associated with the activation of genes essential for cell invasion and metastasis. Additionally, we did a window surgery to determine the behavior of hypoxic and non-hypoxic mouse mammary cancer cells in mice. We have successfully identified tumor cells expressed HIF-GFP and RFP under in vivo window image. Finally, we demonstrated that hypoxia and activating HIF-1 downregulate the DNA mismatch repair proteins (mlh1 and/or msh2), a group of important proteins for maintaining genetic stability. We think that our research discoveries are a major advance in not only understanding the basic biology of cancer but in also developing new insights into the mechanisms of invasion which cause the highest levels of morbidity and mortality among breast cancer patients.


Descriptors :   *HYPOXIA , PROTEINS , BREAST CANCER , CELLS(BIOLOGY) , MAMMARY GLANDS , METASTASIS , FLUORESCENCE , GENES


Subject Categories : Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE