Accession Number : ADA486515


Title :   Role of Crk Adaptor Proteins in Cellular Migration and Invasion in Human Breast Cancer


Descriptive Note : Annual summary rept. 1 Mar 2007-28 Feb 2008


Corporate Author : MCGILL UNIV MONTREAL (QUEBEC)


Personal Author(s) : Fathers, Kelly E


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a486515.pdf


Report Date : Mar 2008


Pagination or Media Count : 51


Abstract : The Crk adaptor proteins (CrkI, CrkII and CrkL) play an important role during cellular signalling by mediating the formation of protein-protein complexes and are involved in cellular migration, invasion, and adhesion. Targeting CrkI and CrkII in breast cancer cell lines by RNA interference demonstrated that loss of Crk expression corresponded with a significant decrease in cell migration and invasion. This implies that Crk adaptor proteins play an important role in integrating signals for migration and invasion of highly malignant cancer cell lines. As migration and invasion are important components of the metastatic cascade, future work includes performing in vivo metastasis assays using cells with stably knocked down expression of Crk proteins. Furthermore, mouse models over-expressing CrkI/II result in delayed ductal outgrowth. MMTV-CrkII mice display enhanced branching and leads to tumour development. This has important implications as we have shown elevated levels of Crk are observed in human breast cancer. This project may provide information, which could be used to develop effective treatments for breast cancer, as well as other cancer types.


Descriptors :   *PROTEINS , *BREAST CANCER , MIGRATION , RIBONUCLEIC ACIDS , MAMMARY GLANDS , METASTASIS , CANADA , EPITHELIUM , CELLS(BIOLOGY)


Subject Categories : Biochemistry
      Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE