Accession Number : ADA482641


Title :   Role of Reactive Stroma in Prostate Cancer Progression


Descriptive Note : Final rept. 12 Jan 2004-11 Jan 2008


Corporate Author : BAYLOR COLL OF MEDICINE HOUSTON TX


Personal Author(s) : Rowley, David R


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a482641.pdf


Report Date : Feb 2008


Pagination or Media Count : 31


Abstract : The purpose of this project is to determine the role of FGF receptor 1 in reactive stroma during prostate tumorigenesis. We are using a novel approach to target transgene expression specifically to the reactive stroma of experimental prostate cancer. Using a modified approach, we are placing an inducible Cre recombinase behind the FAP gene promoter to target expression to reactive stroma. We will cross this mouse with Fgfr1flox mice (LoxP sites flanking FGF receptor 1 alleles). These mice will be crossed with TRAMP mice (prostate cancer model). Induced expression of Cre at sites of reactive stroma generated in the cancer foci will function to excise the FGF receptor 1 alleles and create a conditional knockout mouse. Progression of tumorigenesis in this line of knockout mice will be compared to heterozygous and wild type controls. Progress has been made in each Task. We have completed all cloning steps and have putative founder mice with the FAP-Cre sequence. The Fgfr1flox and have crossed it into the appropriate backgrounds and the TRAMP mice were crossed with the Fgfr1flox mice. This study will pinpoint the role of FGF receptor 1 in reactive stroma promotion of prostate.


Descriptors :   *PROSTATE CANCER , MODELS , NEOPLASMS , TARGETS , CLONES , GENETIC ENGINEERING , WILDLIFE , CANCER , GROWTH(PHYSIOLOGY) , PROSTATE GLAND , PRODUCTION , MICE


Subject Categories : Anatomy and Physiology
      Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE