Accession Number : ADA481903


Title :   Characterization of Reaerosolization in an Effort to Improve Sampling of Airborne Viruses


Descriptive Note : Interim technical rept. 1 Mar 2007-30 Apr 2008


Corporate Author : FLORIDA UNIV GAINESVILLE DEPT OF ENVIRONMENTAL ENGINEERING SCIENCES


Personal Author(s) : Riemenschneider, Lindsey


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a481903.pdf


Report Date : Apr 2008


Pagination or Media Count : 107


Abstract : Airborne virus outbreaks, including the influenza pandemic of 1918, the recent SARS pandemic and the anticipated H5N1 outbreak, plus the perceived threat of bioterrorism warrant concern about the prevalence and potential effects of airborne viruses. However, current bioaerosol sampling methods do not effectively sample airborne viruses (typically 20 - 300 nm). To address this problem, a novel Bioaerosol Amplification Unit (BAU) has been designed and constructed to increase the size of the virus particles by condensational growth, thereby enhancing sample recovery. In this study reaerosolization of viral particles from the impinger was investigated to assess its impact on the capability of the BAU. Reaerosolization was characterized as a function of flow rate and concentration of the collection liquid in the impinger. An impinger containing a known concentration of particles (MS2 bacteriophage or polystyrene latex) was operated at various flow rates with sterile air, and a scanning mobility particle sizer was used to determine the reaerosolization rates. Results indicate that reaerosolization increased with increasing flow rate due to the additional energy added to the system. However, reaerosolization increased with concentration up to 10e4 PFU/mL and then decreased at progressively higher concentrations. This phenomenon likely resulted from aggregation of viral particles or increased surface tension or viscosity at higher concentration. Adjusting surface tension by adding soap and increasing viscosity by adding a layer of heavy white mineral oil decreased reaerosolization. Thus, reaerosolization from an impinger could compromise the improved collection capability of the BAU


Descriptors :   *SAMPLING , *STERILIZATION , *VIRUSES , *AIRBORNE , FLOW RATE , BIOTERRORISM , CONCENTRATION(COMPOSITION) , LATEX , PARTICLES , POLYSTYRENE , VISCOSITY , MOBILITY


Subject Categories : Hygiene and Sanitation
      Microbiology


Distribution Statement : APPROVED FOR PUBLIC RELEASE