Accession Number : ADA481810


Title :   Glutamate Receptor Aptamers and ALS


Descriptive Note : Annual rept. 8 Dec 2006-7 Dec 2007


Corporate Author : STATE UNIV OF NEW YORK AT ALBANY


Personal Author(s) : Niu, Li


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a481810.pdf


Report Date : Jan 2008


Pagination or Media Count : 101


Abstract : Excitotoxicity is one of the leading causes for amyotrophic lateral sclerosis (ALS). Our goal was to develop a novel class of powerful aptamer-based anti-excitotoxic inhibitors against GluR2Qflip a key AMPA receptor subunit that controls the calcium permeability and mediates excitotoxicity. An aptamer is a single-stranded nucleic acid that directly inhibits a protein's function by folding into a specific tertiary structure that dictates high-affinity binding to the target protein. To date we have identified two classes of aptamers (i.e. competitive and noncompetitive aptamers) against GluR2Qflip by using a molecular biology approach called systematic evolution of ligands by exponential enrichment (SELEX). These aptamers are water soluble and have a nanomolar affinity against GluR2Qflip. Their inhibitory properties rival those of any existing small chemical inhibitors. We are continuing to work with these aptamers towards developing them into anti-excitotoxic drugs for treating patients with ALS including those Gulf War veterans suffering from ALS.


Descriptors :   *GLUTAMIC ACID , MOLECULAR BIOLOGY , INHIBITORS , GENETIC DISEASES , TOXINS AND ANTITOXINS , SPINAL CORD , CEREBRAL CORTEX , LIGANDS , RIBONUCLEIC ACIDS


Subject Categories : Genetic Engineering and Molecular Biology
      Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE