Accession Number : ADA478963


Title :   Rapid Active Assay for the Detection of Antibodies to West Nile Virus in Chickens


Descriptive Note : Journal article


Corporate Author : GEORGE MASON UNIV MANASSAS VA NATIONAL CENTER FOR BIODEFENSE AND INFECTIOUS DISEASE


Personal Author(s) : Groves, Stephanie S ; Turell, Michael J ; Bailey, Charles L ; Morozov, Victor N


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a478963.pdf


Report Date : 01 Jan 2008


Pagination or Media Count : 8


Abstract : To reduce the assay time for detecting virus-specific antibodies in serum, we developed microarray-based active immunoassay techniques for detecting West Nile virus (WNV)-specific IgM molecules in chicken blood. The assay uses electrophoretic concentration of IgM molecules onto WNV antigens arrayed on a dialysis membrane followed by detection of bound IgM molecules with functionalized magnetic beads as active labels. This assay takes only 15 minutes and has the same sensitivity as a commercially available human WNV IgM antibody-capture enzyme-linked immunosorbent assay (commonly called a MAC-ELISA) modified for use with chicken sera.


Descriptors :   *ANTIBODIES , *CHICKENS , *WEST NILE VIRUS , MAGNETIC FIELDS , DETECTION , IMMUNOASSAY , ANTIGENS , LABELS , DIALYSIS , BEADS , BLOOD , ASSAYING , MOLECULES , TIME , MEMBRANES


Subject Categories : Biochemistry
      Medicine and Medical Research
      Microbiology


Distribution Statement : APPROVED FOR PUBLIC RELEASE