Accession Number : ADA471901


Title :   DNA Vaccination of the American Crow (Corvus brachyrhynchos) Provides Partial Protection Against Lethal Challenge with West Nile Virus


Descriptive Note : Journal article


Corporate Author : ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FORT DETRICK MD VIROLOGY DIV


Personal Author(s) : Bunning, Michel L ; Fox, Patricia E ; Bowen, Richard A ; Komar, Nicholas ; Chang, Gwong-Jen J ; Speaker, Tully J ; Stephens, Michael R ; Nemeth, Nicole ; Panella, Nicholas A ; Langevin, Stanley A ; Gordy, Paul ; Teehee, Max ; Bright, Patricia R ; Turell, Michael J


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a471901.pdf


Report Date : 01 Jan 2007


Pagination or Media Count : 7


Abstract : The New York 1999 strain of West Nile virus (WNV) is nearly 100% fatal in the American crow (Corvus brachyrhynchos). We evaluated four WNV vaccine formulations in American crows, including intramuscular (i.m.) DNA vaccine, i.m. DNA vaccine with adjuvant, orally administered microencapsulated DNA vaccine, and i.m. killed vaccine. Neutralizing antibodies developed in approximately 80% of crows that received the DNA vaccine i.m. (with or without adjuvant), and in 44% that received the killed vaccine. However, no crows that received the oral microencapsulated DNA vaccine or the placebo developed WNV antibodies. All crows were challenged 10 wk after initial vaccination. No unvaccinated crows survived challenge, and survival rates were 44% (i.m. DNA vaccine), 60% (i.m. DNA vaccine with adjuvant), 0% (oral microencapsulated DNA vaccine), and 11% (killed vaccine). Peak viremia titers in the birds that survived were significantly lower as compared to titers in birds that died. Parenteral administration of a WNV DNA vaccine was associated with reduced mortality but did not provide sterile immunity.


Descriptors :   *BIRDS , *VACCINES , *WEST NILE VIRUS , REPRINTS , FORMULATIONS , DEOXYRIBONUCLEIC ACIDS , IMMUNIZATION , ORAL INTAKE , PROTECTION , ENCAPSULATION , ANTIBODIES


Subject Categories : Biology
      Microbiology
      Pharmacology


Distribution Statement : APPROVED FOR PUBLIC RELEASE