Accession Number : ADA470141


Title :   Identification of Cytoplasmic Proteins Interacting with the Mammary Cell Transforming Domain of Ese-1


Descriptive Note : Annual rept. 29 Mar 2006-28 Mar 2007


Corporate Author : COLORADO UNIV HEALTH SCIENCES CENTER AURORA CO


Personal Author(s) : Gutierrez-Hartmann, Arthur


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a470141.pdf


Report Date : Apr 2007


Pagination or Media Count : 18


Abstract : The Ets family of transcription factors contains several members that are important components of the cellular pathways leading to tumorigenesis. The Ese-1 gene is an Ets member that is particularly relevant to breast cancer. Ese-1 is located on chromosome 1q32.1, in a region that is amplified in 50% of early breast cancers. Ese-1 mRNA is over-expressed in human breast ductal carcinoma in situ (DCIS). We recently discovered that Ese-1 transforms MCF-12A cells via a novel cytoplasmic mechanism in which a unique 40-amino acid (AA), serine- and aspartic acid rich (SAR) domain is necessary and sufficient for transformation. Furthermore, we reported that Ese-1 protein is abundantly expressed in the cytoplasm of human ductal carcinoma in situ (DCIS) specimens. However, identification of the cytoplasmic partners of the Ese-1 SAR motif and the precise mechanism by which cytoplasmic signaling mediated by the Ese-1 SAR motif occurs remain unknown. In this application, we propose to take advantage of this novel paradigm. Specifically, we plan to apply innovative and powerful state-of-the-art molecular yeast two-hybrid and protein biophysical methods (MALDI-TOF and LC-mass spectrometry) to identify proteins that interact with the Ese-1 SAR transformation domain. We will then validate which partners of Ese-1 are functionally relevant to breast cancer. We have made diligent progress, having identified a putative interactor, which we are validating.


Descriptors :   *PROTEINS , *BREAST CANCER , CYTOPLASM , TRANSCRIPTION(GENETICS) , CELLS(BIOLOGY) , MAMMARY GLANDS


Subject Categories : Biochemistry
      Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE