Accession Number : ADA462547


Title :   Biochemical Markers of Brain Injury: An Integrated Proteomics-Based Approach


Descriptive Note : Annual rept. 1 Feb 2005-31 Jan 2006


Corporate Author : FLORIDA UNIV GAINESVILLE


Personal Author(s) : Hayes, Ronald L


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a462547.pdf


Report Date : Feb 2006


Pagination or Media Count : 110


Abstract : Background: Brain injury poses a major problem to military care accounting for 25% of all combat casualties and is the leading cause of death among wounded soldiers reaching Echelon I medical treatment. Incidence of brain injury and resultant long-term disabilities caused by traumatic insults and ischemic events is significantly greater in the civilian population. No clinically useful diagnostic tests exist for traumatic or ischemic brain injury to provide physicians with quantifiable neurochemical markers to help determine the seriousness of the injury the anatomical and cellular pathology of the injury and to guide implementation of appropriate triage and medical management. Study Design: SOW 1 employs integrated proteomics-based technologies to identify specific proteins or peptide fragments in brain released into 0SF and1or blood of rats following experimental traumatic brain injury or focal cerebral ischemia. Technologies include mass spectroscopy 2-D gel electrophoresis phage display of single chain antibodies and antibody chips. SOW 2 employs antibody chips to determine which proteins or peptide fragments released into 0SF following injury are reliably associated with different injury magnitudes and predict changes in histopathological behavioral and electrophysiological outcome measures. SOW 3 develops ELISA-based assays capable of detecting biomarkers in blood. Relevance: Development of objective tnage capabilities for combat medics and1or Echelon I providers would represent a major %eldable breakthrough in the medical management of combat related head trauma.


Descriptors :   *BRAIN , *DIAGNOSIS(MEDICINE) , *PROTEOMICS , WARFARE , ARMY PERSONNEL , NEUROCHEMISTRY , MARKERS , MEDICAL SERVICES , CELLS(BIOLOGY) , BLOOD , TRACER STUDIES , ISCHEMIA , CEREBRUM , INCAPACITATION , TRAUMA , HEAD(ANATOMY) , DEATH , PATHOLOGY , WOUNDS AND INJURIES , ANTIBODIES , BIOCHEMISTRY , PROTEINS


Subject Categories : Biochemistry
      Anatomy and Physiology
      Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE