Accession Number : ADA459169


Title :   EGFR Activation by Spatially Restricted Ligands


Descriptive Note : Annual summary rept. 1 Jun 2003-31 May 2006


Corporate Author : PRINCETON UNIV NJ


Personal Author(s) : Clouse, Katherine N ; Goodrich, Jennifer S


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a459169.pdf


Report Date : Jun 2006


Pagination or Media Count : 52


Abstract : Misregulation of Transforming Growth Factor alpha (TGFalpha) and increased Epidermal Growth Factor Receptor (Egfr) activity has been associated with an increased prognosis of breast cancer. During cogenesis in Drosophila melanogaster local Egfr activation by the spatially-restricted TGFalpha-like ligand Gurken (Grk) is required for axis formation in the egg and future embryo. Squid (Sqd) a heterogeneous nuclear ribonucleoprotein (hnRNF) functions in the localization and translational regulation of grk mRNA. The purpose of this project is to identify factors that function with Sqd to produce spatially-restricted Egfr activation. We have integrated genetic and biochemical methods to identify and characterize the following factors that interact with Sqd: (1) an arginine methyltransferase CG6554 which may serve to methylate Sqd in vivo, (2) Hrb27C an hnRNF protein and Ovarian tumor which are required for proper grk expression and (3) Bruno Cup and polyA-binding protein which function together to mediate grk translational regulation.


Descriptors :   *LIGANDS , *MOLECULAR BIOLOGY , *RIBONUCLEIC ACIDS , *DROSOPHILA , *OVARIAN CANCER , *BREAST CANCER , *GENETICS , ACTIVATION , PREDICTIONS , OVARIES , EPIDERMIS , EMBRYOS , IN VIVO ANALYSIS , BIOCHEMISTRY , PROTEINS


Subject Categories : Genetic Engineering and Molecular Biology
      Biology
      Medicine and Medical Research
      Organic Chemistry


Distribution Statement : APPROVED FOR PUBLIC RELEASE