Accession Number : ADA457028


Title :   A Quantitative Electrochemiluminescence Assay for Clostridium perfringens alpha toxin


Descriptive Note : Journal article


Corporate Author : BROOKE ARMY MEDICAL CENTER FORT SAM HOUSTON TX


Personal Author(s) : Merrill, Gerald A ; Rivera, Victor R ; Neal, Dwayne D ; Young, Charles ; Poli, Mark A


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a457028.pdf


Report Date : 10 Aug 2006


Pagination or Media Count : 8


Abstract : Described is a rapid direct sandwich format electrochemiluminescence assay for identifying and assaying Clostridium perfringens alpha toxin. Biotinylated antibodies to C. perfringens alpha toxin bound to streptavidin paramagnetic beads specifically immunoadsorbed soluble sample alpha toxin which subsequently selectively immunoadsorbed ruthenium (Ru)-labeled detection antibodies. The ruthenium chelate of detection antibodies chemically reacted in the presence of tripropylamine and upon electronic stimulation emitted photons (electrochemiluminescence) that were detected by the photodiode of the detector. Elevated toxin concentrations increased toxin immunoadsorption and the specific immunoadsorption of Ru-labeled antibodies to alpha toxin, which resulted in increased dose-dependent electrochemiluminescent signals. The standardized assay was rapid (single 2.5-h coincubation of all reagents), required no wash steps, and had a sensitivity of about 1ng/ml of toxin. The assay had excellent accuracy and precision and was validated in buffer, serum, and urine with no apparent matrix effects.


Descriptors :   *ELECTROLUMINESCENCE , *TOXINS AND ANTITOXINS , *CHEMILUMINESCENCE , *CLOSTRIDIUM PERFRINGENS , ELECTRONICS , ACCURACY , STANDARDIZATION , ANTIBODIES , RUTHENIUM , URINE , PARAMAGNETISM , BEADS , VITAMIN B COMPLEX , CONCENTRATION(CHEMISTRY) , ASSAYING , PHOTODIODES , PHOTONS , CHEMICAL AGENTS , STIMULATION(GENERAL)


Subject Categories : Biochemistry
      Microbiology


Distribution Statement : APPROVED FOR PUBLIC RELEASE