Accession Number : ADA434907


Title :   Temporal Differential Gene Expression in Explanted Human Retinal Pigment Epithelial Cells at 0.5, 1.0, 3.0, 6.0, 12 and 24 Hours Post-Exposure to 1064 nm, 3.6 ns Pulsed Laser Light


Descriptive Note : Technical rept.


Corporate Author : AIR FORCE ACADEMY COLORADO SPRINGS CO


Personal Author(s) : Obringer, John W ; Johnson, Martin


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a434907.pdf


Report Date : May 2005


Pagination or Media Count : 51


Abstract : The use of laser light for targeting devices and weapons has dramatically increased the likelihood that personnel will be exposed to laser energy during military operations. Expanded medical, research, and industrial laser use may lead to excessive risk of exposure of researchers and technicians and also during commercial applications. Further, the nature and importance of the biophysical mechanisms of photon-tissue interaction at such pulse widths and irradiance are not understood at the fundamental cell and molecular level. A human in vitro model for assessing laser-light damage to tissue at the cell and molecular level is desirable for scientific, political and fiduciary reasons. We assessed the sublethal insult to human retinal pigment epithelial cells using a cadaver organ donor explant system for genes differentially expressed 30 min. and 1,3,6,12 and 24 hours post-exposure using gene expression microarray technology (gene chip). It appears that pulse of laser light are sensed and markedly altered gene expression over time. The 120 pulses of 1064 nm light at 280 mJ per square centimeter appeared to induce the cells into cessation cycling. As expected the various genes assayed fluctuated in expression in all conceivable permutations.


Descriptors :   *WEAPONS , *MILITARY OPERATIONS , *LIGHT PULSES , *TARGETING , *IRRADIATION , *LASER DAMAGE , *VISUAL ACUITY , RISK , MODELS , MOLECULAR STATES , BACKSCATTERING , ANGLE OF ATTACK , IN VITRO ANALYSIS , GENES , PERMUTATIONS , BIOPHYSICS


Subject Categories : Anatomy and Physiology
      Lasers and Masers
      Infrared Detection and Detectors


Distribution Statement : APPROVED FOR PUBLIC RELEASE