Accession Number : ADA433381


Title :   A 52 Kilodalton Protein Vaccine Candidate for Francisella tularensis


Descriptive Note : Technical memorandum


Corporate Author : DEFENCE RESEARCH AND DEVELOPMENT SUFFIELD (ALBERTA)


Personal Author(s) : Sikora, C A ; Berger, B J ; Cherwonogrodsky, John W


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a433381.pdf


Report Date : Dec 2004


Pagination or Media Count : 40


Abstract : For identifying Francisella tularensis vaccine candidates, mice were first vaccinated with Brucella abortus O-polysaccharide (OPS) vaccine. These animals were then given 10 LD(sub 50S) of F. tularensis live vaccine strain (LVS). Sixty percent (60%) of the vaccinated mice survived the multiple lethal dose while all the unvaccinated control mice perished. Sera were collected from these surviving mice and used to probe supernatant and cell lysates of live F. tularensis LVS cultures. Several Francisella tularensis components were identified by this noted antiserum. Mouse serum from mice vaccinated with killed F. tularensis did not identify these components. Of these identified proteins, enzyme digestions and chemical oxidation suggest post-translational modifications for some of the proteins (e.g. a 52 kilodalton (kDa) glycoprotein, a 45 kDa lipoprotein and a 19 kDa nucleoprotein). In low concentrations, the 52 kDa component caused nitrous oxide induction in tissue cultures and in high concentrations it caused cell death. Vaccination with this protein gave mice partial protection (20% survival) from 250 LD(sub 50) of tularemia given intranasally while the addition of other components may have acted synergistically to give enhanced protection (i.e. 100% survival).


Descriptors :   *GLYCOPROTEINS , *VACCINES , *FRANCISCELLA TULARENSIS , *TULAREMIA , HIGH RATE , POLYSACCHARIDES , ENZYMES , PROTEINS , CANADA , OXIDATION , STRAINS(BIOLOGY) , LOW LEVEL , IMMUNE SERUMS , MICE , BLOOD SERUM , CELLS(BIOLOGY) , LIPOPROTEINS , IMMUNIZATION , LETHAL DOSAGE , NITROUS OXIDE , BRUCELLA , BRUCELLOSIS , TISSUE CULTURE , LYSIS


Subject Categories : Medicine and Medical Research
      Microbiology
      Pharmacology


Distribution Statement : APPROVED FOR PUBLIC RELEASE