Accession Number : ADA430422
Title : Fate of Nonindigenous, Endospore-Forming Bacteria in Soils. Strategies for Laboratory and Field Investigations
Descriptive Note : Technical rept.
Corporate Author : ENGINEER RESEARCH AND DEVELOPMENT CENTER HANOVER NH COLD REGIONS RESEARCH AND ENGINEERING LAB
Personal Author(s) : Reynolds, Charles M ; Foley, Karen L ; Ringelberg, David B ; Perry, Lawrence B
Report Date : Nov 2003
Pagination or Media Count : 44
Abstract : Persistence of nonindigenous microorganisms released onto soils or into natural environments can have a significant impact on Department of Defense (DoD) operations. An understanding of competition among various microbial communities is necessary to accurately predict the types of microorganisms that will flourish as well as those that will wane under differing environmental scenarios. In the past, soil microbiology was altered with brute-force techniques such as the saturation of a soil with a decontamination agent. An approach that is more feasible for large areas is to alter soil conditions to promote the desired microbial status or to effectively predict their fate in field conditions. Ultimately, the ability to accurately predict the occurrence of a dominant microbial community will be useful both for predicting the fate of pathogens in the environment and for fostering success in the bioremediation of soils and sediments. Bacillus globigii (BO) was selected to investigate the persistence and fate of nonindigenous bacteria released onto soils. We were able to differentiate BO from indigenous bacteria by combining culturing techniques with lipid-based validation. Enrichment on agar plates produced bright orange BO colonies that were clearly distinct from native microorganisms. These data suggest that there is either an initial loss in viability or an inability to recover 10-15% of the BG soon after inoculation onto the soil.
Descriptors : *BACILLUS SUBTILIS , *SOILS , DECONTAMINATION , BACTERIA , BIODEGRADATION , ANTHRAX , WASTE TREATMENT , SOIL TESTS , PATHOGENIC MICROORGANISMS , FIELD CONDITIONS
Subject Categories : Microbiology
Distribution Statement : APPROVED FOR PUBLIC RELEASE