Accession Number : ADA422079


Title :   Malaria Genome Sequencing Project


Descriptive Note : Final rept. 17 Dec 1997-16 Dec 2003


Corporate Author : INSTITUTE FOR GENOMIC RESEARCH ROCKVILLE MD


Personal Author(s) : Gardner, Malcolm J


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a422079.pdf


Report Date : Jan 2004


Pagination or Media Count : 178


Abstract : The objectives of this Cooperative Agreement were: Specific Aim 1, sequence 3.5 Mb of P. falciparum genomic DNA; Specific Aim 2, annotate the sequence; Specific Aim 3, release the information to the scientific community. Two Specific Aims were added to the Cooperative Agreement: Specific Aim 4, sequencing of P. yoelii to 5X coverage; Specific Aim 5, sequencing of P. vivax to 5X coverage. In 2002 the complete genome sequence of P. falciparum was published in Nature in collaboration with the Sanger Institute and Stanford University. A comparative analysis of the genome of the rodent malaria parasite P. yoelii with that of P. Falciparum was also published. The P. vivax genome has been sequenced to lOX coverage and genome closure is underway. Preliminary P. vivax sequence data has been released to the scientific community. Proteomic analyses of several stages of the P. falciparum life cycle by colleagues at the Scripps Research Institute were also supported by this Agreement. In addition, colleagues at the Malaria Program, Naval Medical Research Center have identified 700 genes expressed in P. falciparum sporozoites and 1,200 genes expressed in P. yoelii liver stages. Bioinformatic approaches were used to identify 300 genes encoding potential liver-stage antigens. The P. falciparum, P. yoelii, and P. vivax genome sequences generated by this effort have supported malaria research worldwide.


Descriptors :   *MALARIA , *SPOROZOITES , *MEDICAL SERVICES , *PLASMODIUM FALCIPARUM , *PLASMODIUM VIVAX , CLOSURES , DEOXYRIBONUCLEIC ACIDS , NAVAL RESEARCH , LIFE CYCLES , AGREEMENTS , LIVER , MEDICAL RESEARCH , COOPERATION , SCIENTIFIC ORGANIZATIONS , LIQUID OXYGEN , GENOME


Subject Categories : Biology
      Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE