Accession Number : ADA420130


Title :   Development of Genetic Therapies for the Hemidesmosol Subtypes of Junction Epidermolysis Bullosa


Descriptive Note : Final rept. 1 Nov 1999-31 Oct 2003


Corporate Author : COLUMBIA UNIV NEW YORK


Personal Author(s) : Christiano, Angela M


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a420130.pdf


Report Date : Nov 2003


Pagination or Media Count : 108


Abstract : During the final award period of this project, we focused on creating the model systems for the in vitro gene therapy experiments, in particular, the in vitro skin model. We worked extensively on development of the in vitro skin model, and succeeded in generating recombinant skin between keratinocytes and dermal cells in skin equivalents. To extend these studies and make them more widely applicable for wound care enhancement in blistering diseases as well as in chemically induced wounding, we then went on to develop a model for in vitro epithelial reprogramming, in which we have begun to utilize different epithelial cell types as donor cells, in addition to keratinocytes, specifically, amnion and cornea cells. Collectively, we have shown that the use of gene delivery combined with epithelial cell-skin equivalent models, show significant promise toward developing a cellular 'bandage' for both genetically and chemically induced skin blistering.


Descriptors :   *GENES , *GENETICS , *SKIN DISEASES , ANTIBIOTICS , BLOOD CELLS , BLOOD DONORS , CELLS(BIOLOGY) , COMPUTER PROGRAMMING , EPIDERMIS , EPITHELIUM , HEALING , IN VIVO ANALYSIS , MEMBRANES(BIOLOGY) , OPTIMIZATION , SKIN(ANATOMY) , WOUNDS AND INJURIES


Subject Categories : Anatomy and Physiology
      Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE