Accession Number : ADA398251


Title :   UG311, An Oncofetal Marker Lost with Prostate Cancer Progression


Descriptive Note : Annual rept. 1 Apr 2000-31 Mar 2001


Corporate Author : VIRGINIA UNIV CHARLOTTESVILLE


Personal Author(s) : Sikes, Robert A


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a398251.pdf


Report Date : Apr 2001


Pagination or Media Count : 79


Abstract : The nRNA expression of a paralogous human sequence to UG311, a murine urogenital sinus expressed sequence tag, was found to decrease with androgen independent progression in the LNCaP model of human prostate cancer progression as determined by RNA blotting. Analysis of the sequence of UG311 determined significant homology to a single-stranded nucleic acid binding protein, nmt55. To exclude nmt55 as the gene corresponding to UG3 11, antibodies and cDNAs were acquired. Scope: As members of the single-stranded nucleic acid binding protein family, nmt55 and UG311 may play a role in DNA repair or RNA splicing. Both functions are currently under evaluation for nmt55. Either of these functions is likely to have impact on the progression and potential therapeutic outcome for prostate cancer patients. Improper splicing would lead to entire classes of proteins being disrupted. Loss of DNA repair enzymes would lead to increased genoinic instability and accelerated progression. Major Findings/progress: As outlined in Aim 2, human prostate tissues and cell lines were screened by IHC. RNA blot analysis was performed to compare with the expression of UG311 in the LNCaP progression model. The data indicate that nmt55 expression does not change with stage or grade nor does the expression of nmt55 mRNA resemble the loss of expression previously observed for UC311 in the LNCaP model of prostate cancer progression. Therefore, the goals of aim 1 become more pertinent. To this end current efforts are focusing on the cloning of the UC311 paralog by RACE and phage library screening A lambda ZAP library has been constructed from C4-2 cells to screen for paralogous clones. RACE assays are underway.


Descriptors :   *GENES , *PROSTATE CANCER , TISSUES(BIOLOGY) , ENZYMES , PROTEINS , DEOXYRIBONUCLEIC ACIDS , ANTIBODIES , GENETIC ENGINEERING , CELLS(BIOLOGY) , RIBONUCLEIC ACIDS , GROWTH(PHYSIOLOGY) , PROSTATE GLAND , BACTERIOPHAGES


Subject Categories : Biochemistry
      Genetic Engineering and Molecular Biology
      Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE