Accession Number : ADA387641


Title :   Construction and Characterization of Human Mammary Epithelial Cell Lines Containing Mutations in the p53 or BRCA1 Genes


Descriptive Note : Final rept. 22 Sep 1994-31 Dec 1998


Corporate Author : UTAH UNIV SALT LAKE CITY


Personal Author(s) : White, Raymond L


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a387641.pdf


Report Date : Jan 1999


Pagination or Media Count : 59


Abstract : The overall goal of this project is to identify and characterize the consequences of human mammary epithelial cells (HMEC) that become deficient in normal p53 or BRCA-l gene functions. We have created retroviral vectors which allow us conditionally express the E6/E7 gene of human papillomavirus type 16 (HPVl6), dominant-negative p53 gene, or anti-sense BRCA-l gene. The consequences of transduction of these viral constructs into primary human mammary epithelial cells will be discovered through controlled in vitro comparisons between genetically altered derivatives and their isogenic parent cells. As we proposed in our last report, we are now focused our efforts on microarray-based comparisons to identify breast cancer related genes. During the past year we have successfully implemented the Microarray Spotting and Scanning techniques. This includes development of robust fluorescent labeling and hybridization protocols as well as the preparation and testing of over 23,000 minimally redundant cDNA target samples for deposition on the microarray slides. We have compared expression profiles from several distinct breast cell lines we had created. We believe that this system will provide us critical information to our understanding of early breast carcinogenesis.


Descriptors :   *EPITHELIUM , *MUTATIONS , *GENES , *BREAST CANCER , HUMANS , ENZYMES , TRANSDUCERS , CELLS(BIOLOGY) , CULTURE , GENETICS , MAMMARY GLANDS , SUPPRESSORS , GROWTH(PHYSIOLOGY) , HYBRIDIZATION , RETROVIRUSES , ONCOGENESIS


Subject Categories : Biochemistry
      Genetic Engineering and Molecular Biology
      Anatomy and Physiology
      Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE