Accession Number : ADA381235


Title :   Investigating the Role of Cooperative Interactions Between the Neu Protooncogene and the Other erbB Family Members in Rat Mammary Carcinogenesis


Descriptive Note : Annual rept. 1 Jul 1998-30 Jun 1999


Corporate Author : WISCONSIN UNIV-MADISON


Personal Author(s) : Gould, Michael N ; Watson, Philip


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a381235.pdf


Report Date : Jul 1999


Pagination or Media Count : 19


Abstract : The phenotype of two additional MMTV-neu N transgenic rats has been determined. Females of both of these lines do not display an abnormal phenotype. In contrast, males of line 6500 develop mammary carcinomas with an average latency of 387 days. This phenotype is highly penetrant, with 77.8% and 100% of the transgenic males carcinoma positive at 14 months and 18 months of age, respectively. Among those rats with carcinomas, the average number of carcinomas/rat was 9.6 and 11.7 for 14 months and 18 months, respectively. Nuclease protection assays have shown that 6500 transgenic females overexpress neu within the mammary gland by approximately 10 fold. This demonstrates that the lack of mammary carcinomas in the 6500 females is not due to a lack of neu overexpression in the mammary gland. Experiments are in progress to investigate the mechanisms underlying the male-specific mammary carcinogenesis in line 6500. In additional experiments, retro viral vectors were used to direct expression of EGFR, erbB3, or erbB4 in the mammary gland of 6500 transgenic and non-transgenic females. None of these retro viral constructs resulted in mammary carcinomas for transgenic or non-transgenic females.


Descriptors :   *FAMILY MEMBERS , *MAMMARY GLANDS , *BREAST CANCER , *ONCOGENESIS , RATS , INTERACTIONS , PROTECTION , ASSAYING , FEMALES , NUCLEASE


Subject Categories : Anatomy and Physiology
      Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE