Accession Number : ADA376289
Title : Insecticide Exposure in Parkinsonism.
Descriptive Note : Annual rept. 1 Jan-31 Dec 99
Corporate Author : VIRGINIA POLYTECHNIC INST AND STATE UNIV BLACKSBURG
Personal Author(s) : Bloomquist, Jeffrey R
Report Date : Jan 2000
Pagination or Media Count : 39
Abstract : A series of behavioral, neurochemical, and immunocytochemical studies were undertaken to characterize the possible role of insecticide exposure in the etiology of Parkinson's disease as it may relate to Gulf War Syndrome. The insecticides under study are the organophosphorus compound chlorpyrifos and the pyrethroid, permethrin given 3 times over a two week period by injection (chlorpyrifos subcutaneous and permethrin intraperitoneal). Permethrin showed an up-regulation of dopamine transport at a dose of 1.5 mg/kg, which is at least two orders of magnitude below its LD50. At higher doses of permethrin (-/25 mg/kg), the increase in transport declined to a level below that of control. Toxic effects may have been involved, since immunocytochemical labeling of the caudate-putamen found that transporter staining was near control levels. Both permethrin and chlorpyrifos caused small, but statistically significant decreases in mitochondrial dehydrogenase activity. However, cytotoxicity was not reflected in levels of striatal dopamine, which were not changed by 100 mg/kg chlorpyrifos or 200 mg/kg permethrin. There was, however, an increase in dopamine turnover at 100 mg/kg chlorpyrifos, as indicated by a significant increase in titers of the dopamine metabolite, 3,4-dihydroxyphenylacetic acid. Chlorpyrifos at 25-100 mg/kg caused 15-84% inhibition of acetylcholinesterase, which correlated reasonably well with effects on open field, rearing, and pole climbing behaviors. Permethrin caused a significant decline in open field behavior, which may have been related to a doubling of muscarinic receptor density in the striatum of mice treated with -/50 mg/kg. These studies demonstrate significant effects on dopamine neurochemistry by these insecticides.
Descriptors : *TOXICITY , *NERVOUS SYSTEM DISEASES , *NEUROCHEMISTRY , CONTROL , DENSITY , GULFS , SIGNS AND SYMPTOMS , ETIOLOGY , BEHAVIOR , RECEPTION , EXPOSURE(PHYSIOLOGY) , INSECTICIDES , DOPAMINE , IMMUNIZATION , FIELD CONDITIONS , METABOLITES , CYTOCHEMISTRY , MUSCARINE , DEHYDROGENASES , MITOCHONDRIA
Subject Categories : Medicine and Medical Research
Distribution Statement : APPROVED FOR PUBLIC RELEASE