Accession Number : ADA346327
Title : Acoustic Microsensors III. Direct Detection of Staphylococcal Enterotoxin B Employing a Piezoelectric Crystal Immunosensor with a Flexible Carboxylated Dextran Matrix as the Biochemical Interface.
Descriptive Note : Final rept.,
Corporate Author : PRINS MAURITS LABORATORIUM TNO RIJSWIJK(NETHERLANDS)
Personal Author(s) : Harteveld, J. L.
Report Date : MAR 1998
Pagination or Media Count : 25
Abstract : Within the framework of the project 'Acoustic Microsensors' (A93KL448) the possibilities of a biosensor system based on an immunological reaction on a piezoelectric crystal sensor are investigated at the TNO Prins Maurits Laboratory (TNO-PML). The results described are a continuation of previous reported work in which the toxin Staphylococcal Enterotoxin B (SEB) was detected in a competition assay with a preincubation period of at least 20 minutes and a detection limit of 100 ng/ml. A new biochemical interface based on a flexible carboxylated dextran matrix, to which antibodies are bound, was developed. With this biosensor system SEB could be detected directly without a preincubation step at a lowest concentration of approximately 7 ng/ml. The biosensor can be easily regenerated by a one-minute injection of 0.1 M HCl.
Descriptors : *DETECTION , *PIEZOELECTRIC CRYSTALS , *ENTEROTOXINS , *TOXINS AND ANTITOXINS , *STAPHYLOCOCCUS , DETECTORS , NETHERLANDS , INTERFACES , BIOCHEMISTRY , ANTIBODIES , BIOLOGICAL DETECTION , RESPONSE(BIOLOGY) , IMMUNOLOGY.
Subject Categories : TOXICOLOGY
Distribution Statement : APPROVED FOR PUBLIC RELEASE