Accession Number : ADA345446
Title : Factors Modulating Estrogen Receptor Activity
Descriptive Note : Annual rept. 1 Jul 96-30 Jun 97
Corporate Author : NEW YORK UNIV MEDICAL CENTER NY
Personal Author(s) : Garabedian, Michael J.
Report Date : JUL 1997
Pagination or Media Count : 16
Abstract : The goal of this project is to elucidate the mechanism of estrogen receptor (ER) action through the identification of Proteins involved in ER signaling using a genetic approach. We have utilized the phenotype of a mutant ER (G4O0V ER), deficient in its ability to bind hormone, as the basis for a dosage suppression screen in yeast. Screening a high-copy yeast genomic library, we have isolated a gene that dramatically suppresses the phenotype of G4OOV ER by increasing its ability to bind hormone. Overexpression of this protein also increases the activity of both wild type estrogen and glucocorticoid receptors (GR). The suppressor is the yeast homologue of the human p23 protein, a component of the molecular chaperonin complex bound to many unliganded steroid receptors.
Descriptors : *ESTROGENS , *BREAST CANCER , HORMONES , PROTEINS , MUTATIONS , IDENTIFICATION , DOSAGE , YEASTS , CYTOPLASM , GENETICS , RECEPTOR SITES(PHYSIOLOGY) , MAMMARY GLANDS , SUPPRESSORS , STEROIDS.
Subject Categories : BIOCHEMISRTY
GENETIC ENGINEERING AND MOLECULAR BIOLOGY
MEDICINE AND MEDICAL RESEARCH
Distribution Statement : APPROVED FOR PUBLIC RELEASE