Accession Number : ADA345446


Title :   Factors Modulating Estrogen Receptor Activity


Descriptive Note : Annual rept. 1 Jul 96-30 Jun 97


Corporate Author : NEW YORK UNIV MEDICAL CENTER NY


Personal Author(s) : Garabedian, Michael J.


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a345446.pdf


Report Date : JUL 1997


Pagination or Media Count : 16


Abstract : The goal of this project is to elucidate the mechanism of estrogen receptor (ER) action through the identification of Proteins involved in ER signaling using a genetic approach. We have utilized the phenotype of a mutant ER (G4O0V ER), deficient in its ability to bind hormone, as the basis for a dosage suppression screen in yeast. Screening a high-copy yeast genomic library, we have isolated a gene that dramatically suppresses the phenotype of G4OOV ER by increasing its ability to bind hormone. Overexpression of this protein also increases the activity of both wild type estrogen and glucocorticoid receptors (GR). The suppressor is the yeast homologue of the human p23 protein, a component of the molecular chaperonin complex bound to many unliganded steroid receptors.


Descriptors :   *ESTROGENS , *BREAST CANCER , HORMONES , PROTEINS , MUTATIONS , IDENTIFICATION , DOSAGE , YEASTS , CYTOPLASM , GENETICS , RECEPTOR SITES(PHYSIOLOGY) , MAMMARY GLANDS , SUPPRESSORS , STEROIDS.


Subject Categories : BIOCHEMISRTY
      GENETIC ENGINEERING AND MOLECULAR BIOLOGY
      MEDICINE AND MEDICAL RESEARCH


Distribution Statement : APPROVED FOR PUBLIC RELEASE