Accession Number : ADA328886


Title :   Development of the Zebra Danio Model: Carcinogenesis and Gene Transfer Studies


Descriptive Note : Final rept. 12 Aug 91-11 Aug 96


Corporate Author : OREGON STATE UNIV CORVALLIS


Personal Author(s) : Hendricks, Jerry D


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a328886.pdf


Report Date : Sep 1996


Pagination or Media Count : 337


Abstract : Zebrafish (Brachydanio rerio) were evaluated as a small fish model for environmental carcinogenesis monitoring. Criteria used for evaluation included: (1) sensitivity to dose response exposures of 6 known carcinogens by 4 routes of exposure, (2) histopathologic evaluation of the resulting lesions and comparison with responses in other species, (3) response to promoters of neoplasia, (4) ability to conduct carcinogen metabolism studies to understand mechanisms of action, (5) determining the role of oncogenes in zebrafish carcinogenesis, and (6) success in developing transgenics that would increase the species' usefulness as a carcinogen monitoring model. Findings were (1) zebrafish did respond to known carcinogens with variable but overall acceptable sensitivity, (2) the histopathology of their responses was similar to that observed in other small fish species, (3) they did not respond well in limited promotional studies, (4) metabolism studies were very difficult due to small fish and organ size and results did not always support observed tumor responses, (5) ras and p53 genes were sequenced but lack of grossly observable tumors prevented the determination of mutations in these genes, and (6) although progress was made, the production of transgenic fish was not achieved. Although none of these results would disqualify zebrafish as environmental monitors, their strict requirement for tropical or subtropical water temperatures would make them completely unsuitable for temperate water use.


Descriptors :   *GENES , *TRANSFER , *ONCOGENESIS , ENVIRONMENTS , MODELS , MONITORING , WATER , NEOPLASMS , METABOLISM , MUTATIONS , TROPICAL REGIONS , CARCINOGENS , GENETICS , FISHES , HISTOPATHOLOGY , ORGANS(ANATOMY) , TEMPERATE REGIONS , SUBTROPICAL REGIONS


Subject Categories : Genetic Engineering and Molecular Biology
      Biology
      Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE