Accession Number : ADA280199


Title :   Production of Antigens and Antibodies for Diagnosis of Arbovirus Diseases.


Descriptive Note : Annual rept. 1 Apr 88-31 Mar 89,


Corporate Author : YALE UNIV NEW HAVEN CT SCHOOL OF MEDICINE


Personal Author(s) : Shope, Robert E


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a280199.pdf


Report Date : 20 May 1994


Pagination or Media Count : 6


Abstract : Antigens and antibodies were produced and standardized for use in the ELISA. Antigens were produced by sucrose-acetone extraction of suckling mouse brain for Germiston, Qalyub, Sicilian, vesicular stomatitis Indiana, and Ganjam viruses. The antigens were inactivated with beta-propiolactone. Rabbits were immunized successfully intravenously with Ross River, Germiston, and Japanese encephalitis viruses using immunogens grown in RK-13 rabbit kidney cells. These animals were boosted 2 months after the initial immunization. Titers in ELISA for optimal coating of the solid phase ranged between 1:500 and 1:64000. Antigens, Antibodies, Arbovirus, Enzyme linked immunosorbent assay


Descriptors :   *DIAGNOSIS(MEDICINE) , *ANTIBODIES , *ANTIGENS , *INFECTIOUS DISEASES , BRAIN , CELLS , ENZYMES , ANIMALS , ACETONES , JAPANESE ENCEPHALITIS , ARBOVIRUSES , IMMUNOGENS , INDIANA , VESICULAR STOMATITIS , KIDNEYS , SOLID PHASES , RABBITS , IMMUNIZATION , SUCROSE , PROPIOLACTONES , HERPETIC VIRUSES , JAPANESE ENCEPHALITIS VIRUSES , ENCEPHALITIS , VIRUSES , RIVERS , EXTRACTION , SOLIDS , PHASE , COATINGS


Subject Categories : Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE