Accession Number : ADA280115


Title :   Effect of Hyperbaric Carbon Dioxide on Spores and Vegetative Cells of Bacillus stearothermophilus


Descriptive Note : Final rept. 30 Mar-30 Sep 1992


Corporate Author : ARMY NATICK RESEARCH DEVELOPMENT AND ENGINEERING CENTER MA


Personal Author(s) : Roskey, Chester T ; Sikes, Anthony


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a280115.pdf


Report Date : May 1994


Pagination or Media Count : 38


Abstract : Spore suspensions in water, 0.05M pH 7 phosphate buffer, 0.05M pH 4.5 and 0.05M pH 4.0 acetate buffer, and antibiotic assay media supplement broth (AAMS) were subjected to 50 psi to 1100 psi carbon dioxide at various temperatures in Parr bombs for up to 96 hours. Spores were resistant to high pressure carbon dioxide treatment at each pH and at both high and low temperatures. Spore suspensions that were first heat activated and then incubated at 550C for a short time increased sensitivity to destruction by CO2. Although spores were resistant to destruction by hyperboric CO2 low pressure (50 psi) CO2 was seen to completely inhibit spore germination and outgrowth. Inhibition to germination was found to be reversible. Spore germination was found to be impaired in carbonated AAMS broth. However, the addition of 50 mM naHCO3 to AAMS enhanced germination and outgrowth. Vegetative cells, Bacillus stearothermophilus, Carbon dioxide, Thermophilic bacteria, Spores, Thermophilic Spoilage, Shelf life, Perishable commodities.


Descriptors :   *CARBON DIOXIDE , *HYPERBARIC CONDITIONS , *HYPERBARIC MEDICINE , *SPORES , ACETATES , ADDITION , ANTIBIOTICS , BACILLUS , BACTERIA , BOMBS , BUFFERS , CARBON , CELLS , COMMODITIES , DESTRUCTION , DIOXIDES , GERMINATION , HEAT , HIGH PRESSURE , INHIBITION , LOW PRESSURE , MEDIA , PHOSPHATES , PRESSURE , REVERSIBLE , SENSITIVITY , SHELF LIFE , TEMPERATURE , THERMOPHILIC FUNGUS , TIME , VEGETATION , WATER


Subject Categories : Biology
      Medicine and Medical Research
      Inorganic Chemistry


Distribution Statement : APPROVED FOR PUBLIC RELEASE