Accession Number : ADA270012


Title :   Immunochemical Characterization of Anti-Acetylcholinesterase Inhibitory Monoclonal Antibodies


Corporate Author : WALTER REED ARMY INST OF RESEARCH WASHINGTON DC DIV OF BIOCHEMISTRY


Personal Author(s) : Gentry, Mary K ; Saxena, Ashima ; Ashani, Yacov ; Doctor, Bhupendra P


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a270012.pdf


Report Date : Jan 1993


Pagination or Media Count : 8


Abstract : Monoclonal antibodies (mAbs) were prepared against native of DFP-inhibited Torpedo california acetylcholinesterase and native or DFP-, MEPQ-, and soman-inhibited fetal bovine serum acetylcholinesterase. The cross reactivity of these antibodies with acetylcholinesterases from various species and their ability to inhibit catalytic activity were determined. Eight antibodies were found to inhibit catalytic activity of either Torpedo or fetal bovine serum enzyme. In all cases the antibodies bound to the native form of the enzymes and in some cases even to the denatured form. None of the antibodies recognized human or horse serum butyrylcholinesterase. Sucrose density gradient centrifugation of enzyme-antibody complexes provided two types of profiles, one with multiple peaks, indicating numerous complexes between tetrameric forms of the enzyme, and the other with single peaks, demonstrating complex formation within the tetrameric form. Different antibodies appeared to interact with slightly different regions, but in all cases the binding encompassed the peripheral anionic site. Decrease in catalytic activity of the enzyme was most likely caused by conformational changes in the enzyme molecule resulting from interaction with these mAbs. Monoclonal antibodies, Anti-cholinesterase, Inhibition, Fetal bovine serum acetylcholinesterase, Torpedo california acetylcholinesterase. cholinesterase


Descriptors :   *ENZYMES , *MONOCLONAL ANTIBODIES , *BOVINES , *CHOLINESTERASE INHIBITORS , *ACETYLCHOLINESTERASE , REPRINTS , DENSITY , MEMBRANES(BIOLOGY) , TORPEDOES , REACTIVITIES , PROFILES , CATALYSIS , SUCROSE , HORSES , GRADIENTS , CHOLINESTERASE , INHIBITION , CALIFORNIA , ANTIBODIES , REGIONS , SITES , HUMANS , INTERACTIONS , MOLECULES


Subject Categories : Biochemistry
      Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE