Accession Number : ADA268162


Title :   Advanced Research Training in Marine Molecular Biology and Biotechnology


Descriptive Note : Final rept. 1 May 90-30 Apr 92,


Corporate Author : DUKE UNIV BEAUFORT NC MARINE LAB


Personal Author(s) : Van Beneden, Rebecca J


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a268162.pdf


Report Date : 02 Aug 1993


Pagination or Media Count : 5


Abstract : The ONR sponsored grant entitled Advanced Research Training in Marine Molecular Biology and Biotechnology founded a training course to teach students and established scientists the basic principles of modern recombinant DNA technology. The emphasis was on the development of marine models and problems in environmental and ocean sciences. Students were trained in the isolation and characterization of DNA and RNA which included Southern and Northern blotting, DNA sequencing and cDNA libraries. other techniques taught were the use of the polymerase chain reaction (PCR), in situ hybridization, gene transfer, mtDNA analysis and immunoblotting. These techniques were applied to problems in environmental toxicology, marine microbiology, the development of transgenic organisms, evolution and population studies, developmental biology and gene expression analysis.


Descriptors :   *MOLECULAR BIOLOGY , *BIOTECHNOLOGY , BIOLOGY , MODELS , STUDENTS , TRAINING , GENES , TRANSFER , LIBRARIES , GRANTS , HYBRIDIZATION , SCIENTISTS , TOXICOLOGY , MICROBIOLOGY , CHAIN REACTIONS , RIBONUCLEIC ACIDS , GENETIC ENGINEERING , OCEANS , CELL DIVISION , ISOLATION , POPULATION , DEOXYRIBONUCLEIC ACIDS , CHAINS


Subject Categories : Biology


Distribution Statement : APPROVED FOR PUBLIC RELEASE