Accession Number : ADA267689


Title :   Simultaneous Metabolism of Chloro- and Methyl-Aromatic Compounds by Selected Bacterial Strains


Descriptive Note : Final rept. 20 Aug 91-19 Aug 92,


Corporate Author : CALIFORNIA UNIV RIVERSIDE


Personal Author(s) : Focht, Dennis D


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a267689.pdf


Report Date : 27 May 1993


Pagination or Media Count : 7


Abstract : Microorganisms are frequently able to degrade anthropogenic materials using pathways that evolved for the assimilation of related naturally-occurring compounds. Complications can arise, however, during the metabolism of mixtures when incompatible intermediates are formed from different components. The breakdown of chloro- and methyl-aromatics, for example, produces catechols which are oxidized differently: chlorocatechols are normally cleaved by ortho fission and methylcatechols by meta fission. If both systems act simultaneously, suicide substrates or dead-end metabolites are usually formed. Nevertheless, bacteria differ in their, ability to cope with such mixtures. A unique bacterium, Pseudomonas cepacia MB2 was isolated by selective enrichment on 2- methylbenzoate, yet was also able to fortuitously utilize 3-chloro-2- methylbenzoate as a sole carbon source. This strain is unique in its ability to utilize an aromatic acid containing both a methyl and chloro substituent via the meta-fission pathway without the production of suicidal products.


Descriptors :   *METABOLISM , *STRAINS(BIOLOGY) , *MICROORGANISMS , *PSEUDOMONAS , PRODUCTION , BACTERIA , MATERIALS , INFECTIOUS DISEASES , ENRICHMENT , GROWTH(PHYSIOLOGY) , FISSION , HYDROLASES , ACIDS , METABOLITES , ASSIMILATION , OXYGEN , MIXTURES , SUBSTRATES , CARBON


Subject Categories : Biochemistry
      Pharmacology


Distribution Statement : APPROVED FOR PUBLIC RELEASE