Accession Number : ADA267113


Title :   Solid-Phase Antibody Capture Hemadsorption Assay for Detection of Hepatitis A Virus Immunoglobulin M Antibodies


Corporate Author : WALTER REED ARMY INST OF RESEARCH WASHINGTON DC


Personal Author(s) : Summers, P L ; DuBois, D R ; Cohen, W H ; Macarthy, P W ; Binn, L N


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a267113.pdf


Report Date : 25 Feb 1993


Pagination or Media Count : 5


Abstract : A solid-phase antibody capture hemadsorption (SPACH) assay was developed to detect hepatitis A virus (HAV)-specific immunoglobulin M (IgM) antibodies in sera from humans recently infected with hepatitis. The assay is performed with microtiter plates coated with anti-human IgM antibodies to capture IgM antibodies from the test sera. HAV-specific IgM antibody is detected by the addition of HAV hemagglutinating antigen and goose erythrocytes. Hemadsorption of erythrocytes to antigen-antibody complexes attached to the solid phase indicate the presence of tgM antibodies. The SPACH assay was compared to a commercial radioimmunoassay and was found to be equally or more sensitive and specific for the detection of HAV IgM antibodies. The SPACH assay is an alternative, rapid assay that does not require hazardous substrates or radioactivity for the detection of HAV-specific antibodies....Hepatitis A, Virus, Antibodies


Descriptors :   *ANTIBODIES , *IMMUNOASSAY , *IMMUNOGLOBULINS , *HEPATITIS VIRUSES , TEST AND EVALUATION , REPRINTS , DETECTION , HUMANS , SOLID PHASES , INFECTIOUS HEPATITIS VIRUS , RADIOIMMUNOASSAY , SERODIAGNOSIS , ANTIGENS , ERYTHROCYTES , VIRUS DISEASES , ENZYMES , PLATES , RADIOACTIVITY


Subject Categories : Medicine and Medical Research
      Microbiology


Distribution Statement : APPROVED FOR PUBLIC RELEASE