Accession Number : ADA267066


Title :   Photoprotein Aequorin Structure Determination by NMR Spectroscopy


Descriptive Note : Final rept. 15 Apr-31 Dec 92,


Corporate Author : CONNECTICUT COLL NEW LONDON DEPT OF CHEMISTRY


Personal Author(s) : Branchini, Bruce R


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a267066.pdf


Report Date : 09 Jul 1993


Pagination or Media Count : 5


Abstract : The nine month grant period was used to acquire experience making sequence specific assignments of proteins using homo- and heteronuclear 2D-NMR. Assignments were made for approximately 95% of the 1H and 15N atoms of Bacillus subtilis HPr phosphorylated at Serine 46. Attempts to carry out preliminary homonuclear 2D-NMR studies on r-aequorin were unsuccessful due to serious solubility problems encountered with a lyophilized sample of the photoprotein which had been charged with benzyl coelenterazine. Arrangements are underway to obtain a suitable NMR sample for preliminary analysis. A brief discussion of the methodology that will be required to obtain a solution structure of r-aequorin is included.


Descriptors :   *PROTEINS , *MOLECULAR STRUCTURE , METHODOLOGY , BACILLUS , THREE DIMENSIONAL , SERINE , BIOLUMINESCENCE , SOLUBILITY , RESONANCE , BACILLUS SUBTILIS , NUCLEAR MAGNETIC RESONANCE


Subject Categories : Biochemistry
      Physical Chemistry


Distribution Statement : APPROVED FOR PUBLIC RELEASE