Accession Number : ADA266834
Title : An Apparatus for Measuring the Biological Oxidation of Hydrogen Gas Under Hyperbaric Conditions.
Descriptive Note : Technical rept.,
Corporate Author : NAVAL MEDICAL RESEARCH INST BETHESDA MD
Personal Author(s) : Kayar, S R ; Goehring, G S ; Long, W E , Jr ; Harabin, A L
Report Date : Jun 1993
Pagination or Media Count : 35
Abstract : We designed, constructed, and operated an apparatus to permit us to expose mammalian tissues to high pressures and concentrations of hydrogen (H2), using the radioisotope tritium (T2) as a label. As part of a study of H2 as a breathing gas for deep diving, we needed to determine if mammalian tissues have a latent capacity to metabolize H2 that is only evident under hyperbaric conditions. The apparatus was designed to store up to 1 Curie T2 between experiments, mix T2 and H2 with air in the experimental chamber without producing an explosive mixture, and reclaim the T2 at the end of the experiment for safe disposal. The T2 was purchased in glass ampules. Storage of T2 was accomplished by placing the T2 ampule in a steel pressure chamber and breaking the ampule to release the T2 under complete containment. The potential problems from explosivity were solved by diluting the T2 and H2 with helium. Reclamation of the T2 was accomplished by passing a controlled stream of the T2 and H2 along with a controlled stream of air through a combustion trap.
Descriptors : *METABOLISM , *HYDROGEN , *OXIDATION , *HYPERBARIC CONDITIONS , *PRESSURE BREATHING , TISSUES(BIOLOGY) , ENZYMES , HELIUM , DISPOSAL , DIVING , LABELS , RECLAMATION , TRITIUM , BREATHING GASES , TRAPS , RADIOACTIVE ISOTOPES , RESPIRATION , STORAGE , RELEASE , COMBUSTION , HIGH PRESSURE , EXPLOSIVES
Subject Categories : Stress Physiology
Escape, Rescue and Survival
Distribution Statement : APPROVED FOR PUBLIC RELEASE