Accession Number : ADA266634


Title :   The Relationship of Metallothionein Induction to the Heat Shock Response


Corporate Author : LETTERMAN ARMY INST OF RESEARCH PRESIDIO OF SAN FRANCISCO CA


Personal Author(s) : Yang, J M ; Bowman, P D ; Deaton, M A ; Schuschereba, S T ; Stuck, B E


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a266634.pdf


Report Date : Apr 1993


Pagination or Media Count : 47


Abstract : Heat shock proteins (hsps) and Metallothioneins (MTs) are sets of proteins specifically synthesized by cells in response to sublethal injury such as heat, heavy metals or radiations. This dissertation investigated the relationship of metallothionein induction to the heat shock response. Sodium arsenite, which induces both hsps and MTs was used to establish a model system for investigation. Electrophoresis conditions that allowed simultaneous identification and quantitation of hsps and MTs were then developed. Identification of MTs was achieved by (35S) cysteine and methionine labeling and (109Cd)-binding. The results of this study indicate that MTs undergo increased synthesis following both heat shock and sodium arsenite treatment. The level of increase of MTs after heat shock was about one-tenth of the increase after sodium arsenite treatment. These results suggest that MTs are heat shock and stress proteins.


Descriptors :   *PROTEINS , *RESPONSE , *SHOCK , *HEAT , STRESSES , RADIATION , MODELS , THESES , CADMIUM , SULFUR , METHIONINE , SUBLETHAL DOSAGE , HEAVY METALS , CYSTEINE , ELECTROPHORESIS , WOUNDS AND INJURIES , METABOLISM , IDENTIFICATION , SODIUM , SYNTHESIS , CELLS , PEPTIDES


Subject Categories : Biochemistry
      Anatomy and Physiology
      Thermodynamics


Distribution Statement : APPROVED FOR PUBLIC RELEASE