Accession Number : ADA266511


Title :   Detection of West Nile Virus by the Polymerase Chain Reaction and Analysis of Nucleotide Sequence Variation


Descriptive Note : Journal article


Corporate Author : NAVAL MEDICAL RESEARCH INST BETHESDA MD


Personal Author(s) : Porter, Kevin R ; Summers, Peter L ; Dubois, Doria ; Puri, Beena ; Nelson, William


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a266511.pdf


Report Date : Jan 1993


Pagination or Media Count : 10


Abstract : A polymerase chain reaction (PCR) assay was developed to rapidly detect and identify West Nile (WN) virus. The RNA from seven isolates of WN virus from six countries and four other flaviviruses (Kunjin, Japanese encephalitis, St. Louis encephalitis, and yellow fever viruses) was reverse- transcribed (RT) and amplified by PCR. The nucleotide sequences of the amplified products were determined by a rapid, automated DNA sequencing method. The WN virus RT/PCR assay detected the target gene segment of isolates from both the African-Middle Eastern group and the Indian group with a sensitivity of approximately 0.05 pg of viral RNA. Kunjin virus was the only other flavivirus tested that produced a band of the appropriate size. Five of seven WN virus isolates showed 9298% homology in the nucleotide sequence of their PCR products. The sequence of one isolate was virtually identical to the published sequence of the Nigerian isolate (99.5% homology). No correlation was established between the degree of nucleotide homology, geographic location, time of isolation, or source of the isolates.


Descriptors :   *DIAGNOSIS(MEDICINE) , *WEST NILE VIRUS , REPRINTS , TEST METHODS , SENSITIVITY , VIRUS DISEASES , ASSAYING , NUCLEOTIDES , SAINT LOUIS ENCEPHALITIS VIRUS , YELLOW FEVER VIRUS , JAPANESE ENCEPHALITIS , RIBONUCLEIC ACIDS , ENCEPHALITIS , GENES , DEOXYRIBONUCLEIC ACIDS , SEQUENCES


Subject Categories : Biochemistry
      Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE