Accession Number : ADA266503


Title :   Release of Iron from Hemoglobin


Corporate Author : LETTERMAN ARMY INST OF RESEARCH PRESIDIO OF SAN FRANCISCO CA DIV OF BLOOD RESEARCH


Personal Author(s) : Panter, S S


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a266503.pdf


Report Date : 17 Feb 1993


Pagination or Media Count : 26


Abstract : The measurement of free iron in hemoglobin solutions as well as the measurement of total hemoglobin-bound iron are important validation assays for large-scale production of hemoglobin-based oxygen carriers. In addition, the detection of iron released from hemoglobin by oxidation or reduction reactions provides information about the stability of iron in heme. These stability measurements may be important in the characterization of structurally modified hemoglobins and can be conducted using spectrophotometric assays based on ferrozine, a chelator of ferrous iron. Techniques for the measurement of free iron in hemoglobin solutions, total hemoglobin-bound iron, and iron released from hemoglobin are described in detail.... Hemoglobin, Hemoglobin solutions, Hemoglobin-based oxygen carriers, Blood substitutes, Iron, Oxidation, Reduction, Ferrozine, Chromophores.


Descriptors :   *IRON , *HEMOGLOBIN , *OXIDATION REDUCTION REACTIONS , *BLOOD SUBSTITUTES , MEASUREMENT , STABILITY , CHROMOPHORES , LIPIDS , PROTEINS , REDUCTION , OXYGEN , OXIDATION , SCALE , CHEMICAL REACTIONS , PATHOPHYSIOLOGY , BACTERIAL DISEASES , NUCLEIC ACIDS , SEPSIS


Subject Categories : Biochemistry


Distribution Statement : APPROVED FOR PUBLIC RELEASE