Accession Number : ADA266470
Title : Clinical Trials on Two Additive Solutions for the Post-thaw Preservation of Red Blood Cells Held for Three Weeks at 4 C
Descriptive Note : Final rept.
Corporate Author : LETTERMAN ARMY INST OF RESEARCH PRESIDIO OF SAN FRANCISCO CA DIV OF BLOOD RESEARCH
Personal Author(s) : Moore, G L ; Hess, J R ; Ledford, M E
Report Date : 29 Jan 1993
Pagination or Media Count : 20
Abstract : An optimized additive solution was developed for the post-thaw preservation of red cells which contained adenine, glucose, disodium phosphate, and citrate buffer. This solution, called AS-17, was compared to Miles AS-3 solution in a clinical trial using 40 subjects (N=20 ea). Fresh-frozen red cells were thawed and deglycerolized after 1-18 months and subjected to a second period of storage in either AS-3 or AS-17 solution for as long as 3 weeks at refrigerator temperatures. Both solutions yielded red cells with 24-hour survivals in excess of 75%. The AS-17 solution produced improved pH, P50, and 2, 3-DPG maintenance compared with the AS-3, but both solutions appear adequate for 3 weeks of post-thaw storage.
Descriptors : *BLOOD STORAGE , *BLOOD PRESERVATION , *BLOOD PRESERVATIVES , MAINTENANCE , BUFFERS , TEMPERATURE , SURVIVABILITY , FREEZING , ADDITIVES , THAWING , PRESERVATION , ADENINE , ADENOSINE PHOSPHATES , ERYTHROCYTES , CRYOGENICS , CITRATES , GLUCOSE , PHOSPHATES , GLYCEROLS , BLOOD CELLS
Subject Categories : Biochemistry
Medicine and Medical Research
Distribution Statement : APPROVED FOR PUBLIC RELEASE