Accession Number : ADA266453


Title :   Evaluation of Hemoglobin-Oxygen Equilibrium Binding by Rapid-Scanning Spectrophotometry and Singular-Value Decomposition


Corporate Author : LETTERMAN ARMY INST OF RESEARCH PRESIDIO OF SAN FRANCISCO CA DIV OF BLOOD RESEARCH


Personal Author(s) : Vandegriff, Kim D ; Shrager, Richard I


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a266453.pdf


Report Date : 08 Mar 1993


Pagination or Media Count : 21


Abstract : A review of a new method to measure oxygen equilibrium binding to hemoglobin solutions by rapid-scanning spectrophotometry is presented. State-of- the-art technologies are reviewed and compared. The new method is based on rapid-scanning spectrophotometry that is used to resolve directly unique optical transitions during continuous hemoglobin oxygenation. Mathematical techniques for matrix multicomponent analysis and singular value decomposition (SVD) are described, and spectral artifacts that are resolved by these sensitive techniques are discussed. Hemoglobin-oxygen binding, Rapid-scanning spectrophotometry, Singular value decomposition.


Descriptors :   *OXYGEN , *HEMOGLOBIN , *SPECTROPHOTOMETRY , THERMODYNAMICS , BIOCHEMISTRY , PROTEINS , DECOMPOSITION , BLOOD CELLS , CHEMICAL EQUILIBRIUM , LIGANDS , CHEMICAL REACTIONS , STRUCTURAL ANALYSIS , TEST METHODS


Subject Categories : Biochemistry
      Anatomy and Physiology


Distribution Statement : APPROVED FOR PUBLIC RELEASE