Accession Number : ADA266430
Title : Heat Shock Protein Induction in Human Cells by CO2 Laser Irradiation
Descriptive Note : Final rept.
Corporate Author : LETTERMAN ARMY INST OF RESEARCH PRESIDIO OF SAN FRANCISCO CA
Personal Author(s) : Ferrando, Ronald E ; Schuschereba, Steven T ; Bowman, Phillip D ; Quong, Julie A ; Yang, Janet M ; Stuck, Bruce E
Report Date : 14 Jun 1993
Pagination or Media Count : 54
Abstract : This study investigated the effect of millisecond exposures from a carbon dioxide (CO2) laser on the production of heat shock proteins in human fibroblast cell cultures. Heat shock or stress proteins (hsp) are induced to high levels by heat shock, transition series and heavy metals, amino acid analogs, certain chemicals, and ultraviolet radiation. These proteins are highly conserved throughout nature and their regulation and cellular functions are just beginning to be understood. The production of hsps was monitored by 35S- methionine incorporation into newly synthesized heat shock protein and by immunocytochemistry. It was shown that C02 laser irradiation could induce the production of hsps. The time course of hsp synthesis and localization within cells was also determined. The hsp synthesis produced by the C02 laser was compared to the effects of sodium arsenite and heat. The results of this study indicate that hsp 70 is the major heat shock protein induced by C02 laser radiation and that this response is most likely due to the thermal effects of the laser.
Descriptors : *CELLS , *PROTEINS , *HUMAN BODY , *SHOCK , *IRRADIATION , *CARBON DIOXIDE LASERS , *HEAT , STRESSES , THERMAL PROPERTIES , RADIATION , TRANSITIONS , METHIONINE , CYTOCHEMISTRY , HEAVY METALS , AMINO ACIDS , CULTURE , REGULATIONS , IMMUNOLOGY , FIBROBLASTS , ANALOGS , SODIUM , ULTRAVIOLET RADIATION , PRODUCTION , SYNTHESIS , CHEMICALS
Subject Categories : Biochemistry
Lasers and Masers
Distribution Statement : APPROVED FOR PUBLIC RELEASE