Accession Number : ADA266253


Title :   Antigen-Antibody Analysis in Leishmaniasis


Descriptive Note : Final rept. 15 Oct 1984-14 Jan 1987


Corporate Author : WAKE FOREST UNIV WINSTON-SALEM NC


Personal Author(s) : Kuhn, Raymond E


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a266253.pdf


Report Date : 30 Apr 1990


Pagination or Media Count : 7


Abstract : Studies were performed to identify antigens of various species of New World Leishmania and Trypanosoma cruzi which would be useful in the differential diagnosis of diseases in humans caused by these pathogenic protozoa. Squirrel monkeys (Saimiri sciureus) were infected with Leishmania braziliensis or L. panamensis and/or T. cruzi and the levels and specificity of antibodies to antigens monitored during the course of single or sequential infections. Control sera were obtained from the monkeys prior to initiation of experimental infections. Antibody levels were determined by ELISA and the specificity for specific antigens of antibodies during the course of infections monitored by Western Blot analyses. Antigens of particular interest and potential use in immunodiagnostic assays were identified. Other information on the course of disease in the squirrel monkey model was determined and are discussed in this report.... Immunodiagnostics, Leishmaniasis, Chagas' disease, RA 1.


Descriptors :   *DIAGNOSIS(MEDICINE) , *IMMUNOASSAY , *LEISHMANIASIS , HUMANS , ANTIBODIES , ANTIGENS , PROTOZOA , KINETOPLAST , SQUIRREL MONKEYS , TRYPANOSOMA CRUZI , PARASITIC DISEASES , PATHOGENIC MICROORGANISMS , IMMUNOLOGY , BLOOD SERUM , MEDICAL RESEARCH


Subject Categories : Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE