Accession Number : ADA265659


Title :   Pathogenesis of Campylobacter Fetus Infections: Serum Resistance Associated with High-Molecular-Weight Surface Proteins


Corporate Author : VETERANS ADMINISTRATION MEDICAL CENTER DENVER CO


Personal Author(s) : Blaser, Martin J ; Smith, Paul F ; Hopkins, Janet A ; Heinzer, Ivo ; Bryner, John H


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a265659.pdf


Report Date : Apr 1987


Pagination or Media Count : 12


Abstract : Campylobacter fetus subspecies fetus causes both systemic and diarrheal illnesses. We studied 38 strains of C. fetus isolated from 34 patients; underlying illness was present in eight (89%) of nine patients with only systemic isolates compared with three (20%) of 15 patients with only fecal isolates (P = .002). In a standardized assay of susceptibility to normal human serum, 27 (71%) strains were resistant, six (16%) had intermediate susceptibility, and five (13%) were serum sensitive. Major protein bands migrating a 100 kDa or 125 kDa on polyacrylamide gels were present in all of the 25 serum-resistant strains tested but in only four of seven serum-sensitive isolates of C fetus from humans and animals (P = .007). The presence of these bands was associated with type A lipopolysaccharide. A low-passaged strain, 82- 40, was serum resistant and contained the 100-kDa protein; however, a spontaneous mutant of this strain lacked this band and was serum sensitive. The 100-kDa and 125-kDa proteins of three strains of C fetus were antigenically cross reactive or identical and were exposed on the surface of the C fetus cell. Serum resistance is inherent to most C. fetus isolates from humans and is associated with the presence of cross-reactive surface proteins


Descriptors :   *PATHOGENESIS , *RESISTANCE(BIOLOGY) , REPRINTS , HUMANS , CELLS , BIOCHEMISTRY , PROTEINS , ILLNESS , PATHOGENIC MICROORGANISMS , ENTERIC BACTERIA , LIPOPOLYSACCHARIDES , MICROBIOLOGY , PATHOLOGY , PATIENTS , INFECTIOUS DISEASES , BLOOD SERUM , VIRULENCE , ANTIGENS


Subject Categories : Medicine and Medical Research
      Microbiology


Distribution Statement : APPROVED FOR PUBLIC RELEASE