Accession Number : ADA265642


Title :   The Use of Stimulable Bioluminescence From Dinoflagellates as a Means of Detecting Toxicity in the Marine Environment


Descriptive Note : Professional paper


Corporate Author : NAVAL COMMAND CONTROL AND OCEAN SURVEILLANCE CENTER RDT AND E DIV SAN DIEGO CA


Personal Author(s) : Lapota, D ; Moskowitz, G ; Grovhoug, J


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a265642.pdf


Report Date : Mar 1993


Pagination or Media Count : 3


Abstract : Phytoplankton bioassays have been used as biological tools in assessing environmental contamination. In our laboratory, a simple bioassay has been developed which measures the light output from bioluminescence dinoflagellates for assessment of toxic effects when exposed to a single toxicant or mixture. Successful use of this type of bioassay has provided data on the acute response and has demonstrated the chronic effects, from hours up to 11 days, on dinoflagellate cells of Pyrocystis lunula and Gonyaulax polyedra upon exposure to several metals and storm drain effluent. Dinoflagellate cells were exposed to various concentrations of tributyltin chloride (TBTCI), copper (11) sulfate (CUS04), zinc sulfate (ZnSO4), or storm drain effluent. Stimulable bioluminescence was measured at each test period (3 or 4 h, 24 h, 48 h, 72 h, etc.) following setup for all assays. Cells were kept in the dark for 3 or 4 h prior to testing. Stirring the cells within the chamber stimulated maximum bioluminescence from the dinoflagellates. An IC50 (an estimated concentration that is likely to cause a 50% reduction in light output) was estimated for all assays. The trend of light reduction as a response to increasing dose level of test article was observed in all assays. A reduction in light output was measured from cells exposed to 1.6, 4.2, and 12.8 ug/L TBTCI. The IC50 decreased from 8.5 ug/L at 120 h to 3.0 ug/L at 264 h. The cells exposed to 6.25%, 12.5%, and 25.0% storm drain effluent exhibited a statistically significant (P=0.05) reduction in light output in as little as 3 h exposure.


Descriptors :   *WATER POLLUTION , *PLANKTON , *BIOLUMINESCENCE , *DINOFLAGELLATA , TEST AND EVALUATION , OUTPUT , METALS , REPRINTS , CELLS , COPPER , CHLORIDES , SULFATES , OCEANOGRAPHY , BIOASSAY , PHYTOPLANKTON , DECAY , EFFLUENTS , CONTAMINATION , ZINC , STORMS , RESPONSE , TOXICITY , LIGHT , MIXTURES , REDUCTION


Subject Categories : Biochemistry
      Biological Oceanography
      Water Pollution and Control


Distribution Statement : APPROVED FOR PUBLIC RELEASE