Accession Number : ADA265567


Title :   Rapid Detection of Mycobacteria in Patients with HIV Infection


Descriptive Note : Annual rept. 15 Mar 1992-14 Mar 1993


Corporate Author : ARMED FORCES INST OF PATHOLOGY WASHINGTON DC


Personal Author(s) : Fritzinger, David C


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a265567.pdf


Report Date : 10 May 1993


Pagination or Media Count : 12


Abstract : The Polymerase Chain Reaction (PCR) along with hybridization to chemiluminescent DNA probes was used to detect mycobacteria potentially present in patient specimens from the Mycobacteriology laboratory at Walter Reed Army Medical Center(WRAMC). DNA from the specimens were prepared by two different methods, and used in the PCR amplifications. of the specimens tested 88% were known to contain Mycobacteria. Of these, we were able to detect 20 (54%), though we were able to detec 82% (9 of 11) using a new genus specific primer set. With our M. tuberculosis primers, we were able to detect 21 of 28 samples that were positive by culture results (75%). We are continuing to evaluate these primer sets, as well as trying to design a primer set for M. avium.... RA 1, Mycobacteria, Mycobacterium avium, Mycobacterium tuberculosis, Polymerase Chain Reaction, DNA Probes, Rapid identification.


Descriptors :   *BIOLOGICAL DETECTION , *MYCOBACTERIUM TUBERCULOSIS , PROBES , DEOXYRIBONUCLEIC ACIDS , INFECTIOUS DISEASES , MEDICAL RESEARCH , CULTURES(BIOLOGY) , ACQUIRED IMMUNE DEFICIENCY SYNDROME , HYBRIDIZATION , CHEMILUMINESCENCE


Subject Categories : Biochemistry
      Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE