Accession Number : ADA265512


Title :   NMR Structural Studies on a Nonnatural Deoxyribonucleoside Mediated Recognition of GC Base Pairs in Pyrimidine-Purine-Pyrimidine Triplexes


Descriptive Note : Technical rept.


Corporate Author : CALIFORNIA INST OF TECH PASADENA DIV OF CHEMISTRY AND CHEMICAL ENGINEERING


Personal Author(s) : Radhakrishnan, Ishwar ; Patel, Dinshaw J ; Priestley, E S ; Nash, Huw M ; Dervan, Peter B


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a265512.pdf


Report Date : 01 Jun 1993


Pagination or Media Count : 18


Abstract : As a part of our ongoing efforts to define the structural aspects of unusual pairing alignments in DNA triplexes by Nuclear Magnetic Resonance spectroscopy, we have examined the structural role of a nonnatural deoxyribonucleoside, P1, that has been shown to mediate the recognition of GC base pairs in pyrimidine-purine-pyrimidine DNA triplexes. A qualitative interpretation of the NMR data indicates that this analog of protonated cytosines is readily accommodated in the third strand segment of an intramolecular triplex system. Furthermore, the observed NOE patterns position the imino and amino protons of P1 opposite the Nitrate and Oxyide atome of guanine respectively, consistent with the previously proposed pairing scheme.


Descriptors :   *SPECTROSCOPY , *DEOXYRIBONUCLEIC ACIDS , *PURINES , *HELIXES , *PYRIMIDINES , *GUANINE , HYDROGEN BONDS , PLASMIDS , MOLECULAR STRUCTURE , PHOSPHATES , NUCLEOTIDES , CLEAVAGE , RESIDUES , RECOGNITION , HUMANS , MODELS


Subject Categories : Biochemistry
      Organic Chemistry


Distribution Statement : APPROVED FOR PUBLIC RELEASE